Assistant Laboratory Animal Technician (ALAT) Practice Exam

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Which substance is typically added to animals' drinking water to inhibit the growth of Pseudomonas aeruginosa?

  1. Chlorine

  2. Alcohol

  3. Acid

  4. Sugar

The correct answer is: Acid

The substance that is typically added to animals' drinking water to inhibit the growth of Pseudomonas aeruginosa is an acid. Specifically, acids such as citric acid or phosphoric acid can lower the pH of the water, creating an environment that is less favorable for the growth of this opportunistic pathogen. Pseudomonas aeruginosa thrives in neutral to slightly alkaline conditions. By using acidification as a method of controlling bacterial growth, it can effectively reduce the risk of infection or contamination within the animal facility. This method aligns with best practices in animal husbandry and microbiological control, ensuring that the drinking water remains safe and healthy for the animals. Chlorine, while a common disinfectant, can be harmful to some animal species at certain concentrations and is not typically used in a sustained manner in drinking water for animals. Alcohol is not practical for this purpose because it would be harmful to the animals. Sugar does not have any inhibitory effect on Pseudomonas aeruginosa and could potentially promote microbial growth instead.